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ELK Biotechnology
SKU:ES3629
TP53INP1 Rabbit Polyclonal Antibody
TP53INP1 Rabbit Polyclonal Antibody
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$248.00 USD
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TP53INP1 Rabbit Polyclonal Antibody
Overview
| Product name: | TP53INP1 rabbit pAb |
| Alternative Names: | TP53INP1; P53DINP1; SIP; Tumor protein p53-inducible nuclear protein 1; Stress-induced protein; p53-dependent damage-inducible nuclear protein 1; p53DINP1 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human TP53INP1. AA range:171-220 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 27kD |
| GeneID(Human): | 94241 |
| Human Swiss-Prot No: | Q96A56 |
| Cellular localization: | Cytoplasm, cytosol. Nucleus. Nucleus, PML body. Cytoplasmic vesicle, autophagosome. Shuttles between the nucleus and the cytoplasm, depending on cellular stress conditions, and re-localizes to autophagosomes on autophagy activation. |
| Background: | function:In response to double-strand DNA breaks, promotes p53/TP53 phosphorylation on 'Ser-46' and subsequent apoptosis.,induction:By adriamycin, gamma irradiation and H(2)O(2), in a p53-dependent way. At lower levels by ultraviolet irradiation.,subcellular location:Concentrated in PML/POD/ND10 nuclear bodies.,subunit:Interacts with TP53 and HIPK2.,tissue specificity:Ubiquitously expressed., |
| Species Reactivity: | Human;Rat;Mouse; |
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Western Blot analysis of various cells using TP53INP1 Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN -
Western blot analysis of lysates from COLO205, HUVEC, and HepG2 cells, using TP53INP1 Antibody. The lane on the right is blocked with the synthesized peptide. -
Western blot analysis of the lysates from HT-29 cells using TP53INP1 antibody. -
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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