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ELK Biotechnology
SKU:ES3617
TLK2 Rabbit Polyclonal Antibody
TLK2 Rabbit Polyclonal Antibody
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$248.00 USD
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TLK2 Rabbit Polyclonal Antibody
Overview
| Product name: | TLK2 rabbit pAb |
| Alternative Names: | TLK2; Serine/threonine-protein kinase tousled-like 2; HsHPK; PKU-alpha; Tousled-like kinase 2 |
| Applications: | WB;ELISA;IHC |
| Recommended Dilutions: | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human TLK2. AA range:191-240 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 90kD |
| GeneID(Human): | 11011 |
| Human Swiss-Prot No: | Q86UE8 |
| Cellular localization: | Nucleus. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. Colocalizes with the cytoplasmic intermediate filament system during the G1 phase of the cell cycle. Present in the perinuclear region at S phase and in the nucleus at late G2. |
| Background: | This gene encodes a nuclear serine/threonine kinase that was first identified in Arabidopsis. The encoded protein is thought to function in the regulation of chromatin assembly in the S phase of the cell cycle by regulating the levels of a histone H3/H4 chaperone. This protein is associated with double-strand break repair of DNA damage caused by radiation. Pseudogenes of this gene are present on chromosomes 10 and 17. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013], |
| Species Reactivity: | Human;Mouse |
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Western Blot analysis of various cells using TLK2 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western blot analysis of lysates from COLO cells, using TLK2 Antibody. The lane on the right is blocked with the synthesized peptide. -
Western blot analysis of the lysates from HeLa cells using TLK2 antibody. -
Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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