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ELK Biotechnology
SKU:ES3583
Tetraspanin-8 Rabbit Polyclonal Antibody
Tetraspanin-8 Rabbit Polyclonal Antibody
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Tetraspanin-8 Rabbit Polyclonal Antibody
Overview
| Product name: | Tetraspanin-8 rabbit pAb |
| Alternative Names: | TSPAN8; TM4SF3; Tetraspanin-8; Tspan-8; Transmembrane 4 superfamily member 3; Tumor-associated antigen CO-029 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human TSPAN8. AA range:96-145 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 26kD |
| GeneID(Human): | 7103 |
| Human Swiss-Prot No: | P19075 |
| Cellular localization: | Membrane; Multi-pass membrane protein. |
| Background: | The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This gene is expressed in different carcinomas. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Rat;Mouse; |
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Western Blot analysis of various cells using Tetraspanin-8 Polyclonal Antibody diluted at 1:1000 -
Immunofluorescence analysis of HeLa cells, using TSPAN8 Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from 293 and COLO cells, using TSPAN8 Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human colon cancer. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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