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ELK Biotechnology
SKU:ES3485
Spindlin-1 Rabbit Polyclonal Antibody
Spindlin-1 Rabbit Polyclonal Antibody
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Overview
| Product name: | Spindlin-1 rabbit pAb |
| Alternative Names: | SPIN1; OCR; SPIN; Spindlin-1; Ovarian cancer-related protein |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human SPIN1. AA range:111-160 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 30kD |
| GeneID(Human): | 10927 |
| Human Swiss-Prot No: | Q9Y657 |
| Cellular localization: | Nucleus . Nucleus, nucleolus . |
| Background: | function:May play a role in cell-cycle regulation during the transition from gamete to embryo.,PTM:Phosphorylated during oocyte meiotic maturation.,sequence caution:Translated as Glu.,similarity:Belongs to the SPIN/STSY family., |
| Species Reactivity: | Human;Mouse;Rat |
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Immunofluorescence analysis of Hela cell. 1,Spindlin-1 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Bcl-2 Monoclonal Antibody(6B5)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at -
Western Blot analysis of various cells using Spindlin-1 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western Blot analysis of HeLa cells using Spindlin-1 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absor
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