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ELK Biotechnology
SKU:ES3443
Siah-2 Rabbit Polyclonal Antibody
Siah-2 Rabbit Polyclonal Antibody
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Siah-2 Rabbit Polyclonal Antibody
Overview
| Product name: | Siah-2 rabbit pAb |
| Alternative Names: | SIAH2; E3 ubiquitin-protein ligase SIAH2; Seven in absentia homolog 2; Siah-2; hSiah2 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human SIAH2. AA range:241-290 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 38kD |
| GeneID(Human): | 6478 |
| Human Swiss-Prot No: | O43255 |
| Cellular localization: | Cytoplasm . Nucleus . Predominantly cytoplasmic. Partially nuclear. . |
| Background: | This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in regulating cellular response to hypoxia. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using Siah-2 Polyclonal Antibody -
Western blot analysis of lysates from COLO cells, using SIAH2 Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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