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ELK Biotechnology
SKU:ES3404
SAE1 Rabbit Polyclonal Antibody
SAE1 Rabbit Polyclonal Antibody
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$248.00 USD
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SAE1 Rabbit Polyclonal Antibody
Overview
| Product name: | SAE1 rabbit pAb |
| Alternative Names: | SAE1; AOS1; SUA1; UBLE1A; SUMO-activating enzyme subunit 1; Ubiquitin-like 1-activating enzyme E1A |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human AOS1. AA range:221-270 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 38kD |
| GeneID(Human): | 10055 |
| Human Swiss-Prot No: | Q9UBE0 |
| Cellular localization: | Nucleus . |
| Background: | SUMO1 activating enzyme subunit 1(SAE1) Homo sapiens Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using SAE1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Immunofluorescence analysis of HUVEC cells, using AOS1 Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from 293 cells, using AOS1 Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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