Rho A Rabbit Polyclonal Antibody

Name: Rho A Rabbit Polyclonal Antibody
Brand: ELK Biotechnology
SKU: ES3349
Price: 248.0 USD
Availability: InStock

$248.00 USD

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Rho A Rabbit Polyclonal Antibody

Overview

Product name:	Rho A rabbit pAb
Alternative Names:	RHOA; ARH12; ARHA; RHO12; Transforming protein RhoA; Rho cDNA clone 12; h12
Applications:	IF;WB;IHC;ELISA
Recommended Dilutions:	IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human RhoA. AA range:144-193
Storage:	Rabbit
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	22kD
GeneID（Human）:	387
Human Swiss-Prot No:	P61586
Cellular localization:	Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton. Cleavage furrow. Cytoplasm, cell cortex . Midbody. Cell projection, lamellipodium . Cell projection, dendrite . Nucleus . Localized to cell-cell contacts in calcium-treated keratinocytes (By similarity). Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in an activated form and in a myosin- and actin-independent manner. .
Background:	This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015],
Species Reactivity:	Human;Mouse;Rat

Immunofluorescence analysis of Hela cell. 1,Rho A Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
Immunofluorescence analysis of rat-lung tissue. 1,Rho A Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture
Immunofluorescence analysis of rat-lung tissue. 1,Rho A Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture
Immunofluorescence analysis of mouse-lung tissue. 1,Rho A Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Pictur

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Rho A Rabbit Polyclonal Antibody

Rho A Rabbit Polyclonal Antibody

Overview