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ELK Biotechnology
SKU:ES3334
RBAK Rabbit Polyclonal Antibody
RBAK Rabbit Polyclonal Antibody
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$248.00 USD
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RBAK Rabbit Polyclonal Antibody
Overview
| Product name: | RBAK rabbit pAb |
| Alternative Names: | RBAK; ZNF769; RB-associated KRAB zinc finger protein; RB-associated KRAB repressor; hRBaK; Zinc finger protein 769 |
| Applications: | WB;IF;ELISA;IHC |
| Recommended Dilutions: | WB 1:500-2000; IF/ICC 1:50-200;ELISA 1:2000-20000;IHC-p 1:50-200 |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human RBAK. AA range:1-50 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 85kD |
| GeneID(Human): | 57786 |
| Human Swiss-Prot No: | Q9NYW8 |
| Cellular localization: | Nucleus . |
| Background: | This gene encodes a nuclear protein which interacts with the tumor suppressor retinoblastoma 1. The two interacting proteins are thought to act as a transcriptional repressor for promoters which are activated by the E2F1 transcription factor. This protein contains a Kruppel-associated box (KRAB), which is a transcriptional repressor motif. Read-through transcripts that include exons from the downstream gene LOC389458 are expressed from this locus. [provided by RefSeq, Mar 2011], |
| Species Reactivity: | Human;Mouse |
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Western Blot analysis of various cells using RBAK Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western blot analysis of lysates from HepG2 cells, using RBAK Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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