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ELK Biotechnology
SKU:ES3309
Rad52 Rabbit Polyclonal Antibody
Rad52 Rabbit Polyclonal Antibody
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$248.00 USD
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Rad52 Rabbit Polyclonal Antibody
Overview
| Product name: | Rad52 rabbit pAb |
| Alternative Names: | RAD52; DNA repair protein RAD52 homolog |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human RAD52. AA range:70-119 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 46kD |
| GeneID(Human): | 5893 |
| Human Swiss-Prot No: | P43351 |
| Cellular localization: | Nucleus . |
| Background: | The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014], |
| Species Reactivity: | Human;Mouse |
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Western Blot analysis of various cells using Rad52 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western Blot analysis of 293 cells using Rad52 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Immunofluorescence analysis of COS7 cells, using RAD52 Antibody. The picture on the right is blocked with the synthesized peptide. -
Immunohistochemistry analysis of paraffin-embedded human testis tissue, using RAD52 Antibody. The picture on the right is blocked with the synthesized peptide.
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