Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human Rac1/CDC42. AA range:38-87
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
26kD
GeneID（Human）:
5879/5880/5881/998
Human Swiss-Prot No:
P63000/P15153/P60763/P60953
Cellular localization:
Cell membrane ; Lipid-anchor ; Cytoplasmic side . Melanosome . Cytoplasm . Cell projection, lamellipodium . Cell projection, dendrite . Cell junction, synapse . Nucleus . Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (PubMed:1903399). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Localizes to the lamellipodium in a SH3RF1-dependent manner (By similarity). In macrophages, cytoplasmic location increases upon CSF1 stimulation (By similarity). Activation by GTP-binding promotes nuclear localization (PubMed:12551911). .
Background:
The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009],
Western Blot analysis of various cells using Rac1/2/3/CDC42 Polyclonal Antibody
Western Blot analysis of 3T3 cells using Rac1/2/3/CDC42 Polyclonal Antibody
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Rac1/CDC42 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from NIH/3T3 cells, treated with EGF 200ng/ml 30', using Rac1/CDC42 Antibody. The lane on the right is blocked with the synthesized peptide.