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ELK Biotechnology
SKU:ES3284
PU.1 Rabbit Polyclonal Antibody
PU.1 Rabbit Polyclonal Antibody
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$248.00 USD
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PU.1 Rabbit Polyclonal Antibody
Overview
| Product name: | PU.1 rabbit pAb |
| Alternative Names: | SPI1; Transcription factor PU.1; 31 kDa-transforming protein |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human SPI1. AA range:181-230 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 32kD |
| GeneID(Human): | 6688 |
| Human Swiss-Prot No: | P17947 |
| Cellular localization: | Nucleus . |
| Background: | This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat;Monkey |
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Western Blot analysis of various cells using PU.1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i -
Western blot analysis of lysates from COS7 cells, using SPI1 Antibody. The lane on the right is blocked with the synthesized peptide. -
Western blot analysis of the lysates from HUVECcells using SPI1 antibody.
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