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ELK Biotechnology
SKU:ES3263
PRPF18 Rabbit Polyclonal Antibody
PRPF18 Rabbit Polyclonal Antibody
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$248.00 USD
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PRPF18 Rabbit Polyclonal Antibody
Overview
| Product name: | PRPF18 rabbit pAb |
| Alternative Names: | PRPF18; HPRP18; Pre-mRNA-splicing factor 18; PRP18 homolog; hPRP18 |
| Applications: | WB;ELISA;IHC |
| Recommended Dilutions: | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PRPF18. AA range:182-231 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 40kD |
| GeneID(Human): | 8559 |
| Human Swiss-Prot No: | Q99633 |
| Cellular localization: | Nucleus speckle . Colocalizes with spliceosomal snRNPs. . |
| Background: | Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to the yeast splicing factor Prp18. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using PRPF18 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western Blot analysis of HuvEc cells using PRPF18 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western blot analysis of lysates from 293, MCF-7, and HUVEC cells, using PRPF18 Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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