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ELK Biotechnology
SKU:ES3260
PRIM1 Rabbit Polyclonal Antibody
PRIM1 Rabbit Polyclonal Antibody
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$248.00 USD
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PRIM1 Rabbit Polyclonal Antibody
Overview
| Product name: | PRIM1 rabbit pAb |
| Alternative Names: | PRIM1; DNA primase small subunit; DNA primase 49 kDa subunit; p49 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PRIM1. AA range:371-420 |
| Storage: | -20°C/1 year |
| Storage: | Rabbit |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 50kD |
| GeneID(Human): | 5557 |
| Human Swiss-Prot No: | P49642 |
| Cellular localization: | nucleoplasm,alpha DNA polymerase:primase complex,membrane,primosome complex, |
| Background: | The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using PRIM1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western blot analysis of lysates from COLO and HeLa cells, using PRIM1 Antibody. The lane on the right is blocked with the synthesized peptide. -
Western blot analysis of the lysates from K562 cells using PRIM1 antibody. -
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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