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ELK Biotechnology
SKU:ES3244
POLR3E Rabbit Polyclonal Antibody
POLR3E Rabbit Polyclonal Antibody
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$248.00 USD
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POLR3E Rabbit Polyclonal Antibody
Overview
| Product name: | POLR3E rabbit pAb |
| Alternative Names: | POLR3E; KIAA1452; DNA-directed RNA polymerase III subunit RPC5; RNA polymerase III subunit C5; DNA-directed RNA polymerase III 80 kDa polypeptide |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human RPC5. AA range:191-240 |
| Storage: | -20°C/1 year |
| Storage: | Rabbit |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 80kD |
| GeneID(Human): | 55718 |
| Human Swiss-Prot No: | Q9NVU0 |
| Cellular localization: | Nucleus. |
| Background: | function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters.,subunit:Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). Interacts with POLR3D/RPC4., |
| Species Reactivity: | Human;Mouse |
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Western Blot analysis of various cells using POLR3E Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western Blot analysis of JK cells using POLR3E Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorb -
Immunofluorescence analysis of LOVO cells, using RPC5 Antibody. The picture on the right is blocked with the synthesized peptide.
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