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ELK Biotechnology
SKU:ES3243
POLR3A Rabbit Polyclonal Antibody
POLR3A Rabbit Polyclonal Antibody
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$248.00 USD
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POLR3A Rabbit Polyclonal Antibody
Overview
| Product name: | POLR3A rabbit pAb |
| Alternative Names: | POLR3A; DNA-directed RNA polymerase III subunit RPC1; RNA polymerase III subunit C1; DNA-directed RNA polymerase III largest subunit; DNA-directed RNA polymerase III subunit A; RNA polymerase III 155 kDa subunit; RPC155; RNA polymerase III |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human RPC1. AA range:31-80 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 156kD |
| GeneID(Human): | 11128 |
| Human Swiss-Prot No: | O14802 |
| Cellular localization: | Nucleus. |
| Background: | The protein encoded by this gene is the catalytic component of RNA polymerase III, which synthesizes small RNAs. The encoded protein also acts as a sensor to detect foreign DNA and trigger an innate immune response. [provided by RefSeq, Aug 2011], |
| Species Reactivity: | Human;Mouse;Monkey |
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Western Blot analysis of various cells using POLR3A Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western blot analysis of lysates from COS and HUVEC cells, using RPC1 Antibody. The lane on the right is blocked with the synthesized peptide. -
Western blot analysis of the lysates from 293 cells using RPC1 antibody. -
Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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