Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human PLCG1. AA range:736-785
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
150kD
GeneID（Human）:
5335
Human Swiss-Prot No:
P19174
Cellular localization:
Cell projection, lamellipodium . Cell projection, ruffle . Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment. .
Background:
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Western Blot analysis of various cells using PLC γ1 Polyclonal Antibody diluted at 1:2000
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PLCG1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HepG2, using PLCG1 Antibody. The lane on the right is blocked with the synthesized peptide.