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ELK Biotechnology
SKU:ES3232
Plakophilin 2 Rabbit Polyclonal Antibody
Plakophilin 2 Rabbit Polyclonal Antibody
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$248.00 USD
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Plakophilin 2 Rabbit Polyclonal Antibody
Overview
| Product name: | Plakophilin 2 rabbit pAb |
| Alternative Names: | PKP2; Plakophilin-2 |
| Applications: | WB;ELISA;IHC |
| Recommended Dilutions: | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PKP2. AA range:632-681 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 97kD |
| GeneID(Human): | 5318 |
| Human Swiss-Prot No: | Q99959 |
| Cellular localization: | Nucleus . Cell junction, desmosome . Nuclear and associated with desmosomes. |
| Background: | This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This gene product may regulate the signaling activity of beta-catenin. Two alternately spliced transcripts encoding two protein isoforms have been identified. A processed pseudogene with high similarity to this locus has been mapped to chromosome 12p13. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Rat |
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Western Blot analysis of various cells using Plakophilin 2 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western Blot analysis of HepG2 cells using Plakophilin 2 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western blot analysis of lysates from HeLa and HepG2 cells, using PKP2 Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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