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ELK Biotechnology
SKU:ES3228
PKD1 Rabbit Polyclonal Antibody
PKD1 Rabbit Polyclonal Antibody
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$248.00 USD
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PKD1 Rabbit Polyclonal Antibody
Overview
| Product name: | PKD1 rabbit pAb |
| Alternative Names: | PRKD1; PKD; PKD1; PRKCM; Serine/threonine-protein kinase D1; Protein kinase C mu type; Protein kinase D; nPKC-D1; nPKC-mu |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PKD1/PKC mu. AA range:171-220 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 101kD |
| GeneID(Human): | 5587 |
| Human Swiss-Prot No: | Q15139 |
| Cellular localization: | Cytoplasm . Cell membrane . Golgi apparatus, trans-Golgi network . Translocation to the cell membrane is required for kinase activation. |
| Background: | PRKD1 is a serine/threonine kinase that regulates a variety of cellular functions, including membrane receptor signaling, transport at the Golgi, protection from oxidative stress at the mitochondria, gene transcription, and regulation of cell shape, motility, and adhesion (summary by Eiseler et al., 2009 [PubMed 19329994]).[supplied by OMIM, Nov 2010], |
| Species Reactivity: | Human;Mouse;Rat |
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Immunofluorescence analysis of Hela cell. 1,PKD1 Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min. -
Western Blot analysis of HuvEc cells using PKD1 Polyclonal Antibody -
Immunofluorescence analysis of A549 cells, using PKD1/PKC mu Antibody. The picture on the right is blocked with the synthesized peptide. -
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PKD1/PKC mu Antibody. The picture on the right is blocked with the synthesized peptide.
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