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ELK Biotechnology
SKU:ES3219
PKA Iβ reg Rabbit Polyclonal Antibody
PKA Iβ reg Rabbit Polyclonal Antibody
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PKA Iβ reg Rabbit Polyclonal Antibody
Overview
| Product name: | PKA Iβ reg rabbit pAb |
| Alternative Names: | PRKAR1B; cAMP-dependent protein kinase type I-beta regulatory subunit |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human KAP1. AA range:106-155 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 43kD |
| GeneID(Human): | 5575 |
| Human Swiss-Prot No: | P31321 |
| Cellular localization: | Cell membrane . |
| Background: | The protein encoded by this gene is a regulatory subunit of cyclic AMP-dependent protein kinase A (PKA), which is involved in the signaling pathway of the second messenger cAMP. Two regulatory and two catalytic subunits form the PKA holoenzyme, disbands after cAMP binding. The holoenzyme is involved in many cellular events, including ion transport, metabolism, and transcription. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2015], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using PKA Iβ reg Polyclonal Antibody -
Western Blot analysis of COLO205 cells using PKA Iβ reg Polyclonal Antibody -
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absor -
Western blot analysis of lysates from COLO205 cells, using KAP1 Antibody. The lane on the right is blocked with the synthesized peptide.
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