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ELK Biotechnology
SKU:ES3209
PIG-X Rabbit Polyclonal Antibody
PIG-X Rabbit Polyclonal Antibody
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PIG-X Rabbit Polyclonal Antibody
Overview
| Product name: | PIG-X rabbit pAb |
| Alternative Names: | PIGX; Phosphatidylinositol-glycan biosynthesis class X protein; PIG-X |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PIGX. AA range:183-232 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 26kD |
| GeneID(Human): | 54965 |
| Human Swiss-Prot No: | Q8TBF5 |
| Cellular localization: | Endoplasmic reticulum membrane ; Single-pass type I membrane protein . |
| Background: | This gene encodes a type I transmembrane protein in the endoplasmic reticulum (ER). The protein is an essential component of glycosylphosphatidylinositol-mannosyltransferase I, which transfers the first of the four mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Studies in rat indicate that the protein is translated from a non-AUG translation initiation site. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009], |
| Species Reactivity: | Human;Rat;Mouse; |
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Western Blot analysis of various cells using PIG-X Polyclonal Antibody diluted at 1:1000 -
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i -
Western blot analysis of lysates from 293, COLO, HUVEC, and Jurkat cells, using PIGX Antibody. The lane on the right is blocked with the synthesized peptide.
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