PI 3-kinase p85α/γ Rabbit Polyclonal Antibody

Name: PI 3-kinase p85α/γ Rabbit Polyclonal Antibody
Brand: ELK Biotechnology
SKU: ES3200
Price: 248.0 USD
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PI 3-kinase p85α/γ Rabbit Polyclonal Antibody

Overview

Product name:	PI 3-kinase p85α/γ rabbit pAb
Alternative Names:	PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alph
Applications:	WB;IHC;IF;ELISA
Recommended Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha/gamma. AA range:436-485
Storage:	Rabbit
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	54+83kD
GeneID（Human）:	5295/8503
Human Swiss-Prot No:	P27986/Q92569
Cellular localization:	nucleus,cytoplasm,cis-Golgi network,cytosol,plasma membrane,cell-cell junction,phosphatidylinositol 3-kinase complex,phosphatidylinositol 3-kinase complex, class IA,membrane,perinuclear endoplasmic reticulum membrane,
Background:	Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011],
Species Reactivity:	Human;Mouse;Rat;Monkey;Chicken(testedbyourcustomer）

Immunofluorescence analysis of human-lung tissue. 1,PI 3-kinase p85α/γ Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:T
Immunofluorescence analysis of rat-lung tissue. 1,PI 3-kinase p85α/γ Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Tar
Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,PI 3-kinase p85α/γ Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at

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PI 3-kinase p85α/γ Rabbit Polyclonal Antibody

PI 3-kinase p85α/γ Rabbit Polyclonal Antibody

Overview