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ELK Biotechnology
SKU:ES3181
PFKM Rabbit Polyclonal Antibody
PFKM Rabbit Polyclonal Antibody
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$248.00 USD
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PFKM Rabbit Polyclonal Antibody
Overview
| Product name: | PFKM rabbit pAb |
| Alternative Names: | PFKM; PFKX; 6-phosphofructokinase; muscle type; Phosphofructo-1-kinase isozyme A; PFK-A; Phosphofructokinase-M; Phosphofructokinase 1; Phosphohexokinase |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PFK-1. AA range:320-369 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 85kD |
| GeneID(Human): | 5213 |
| Human Swiss-Prot No: | P08237 |
| Cellular localization: | Cytoplasm . |
| Background: | Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using PFKM Polyclonal Antibody -
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i -
Immunohistochemistry analysis of PFK-1 antibody in paraffin-embedded human brain tissue. -
Western blot analysis of lysate from Hela cells, using PFK-1 antibody.
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