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ELK Biotechnology
SKU:ES3170
PDK2 Rabbit Polyclonal Antibody
PDK2 Rabbit Polyclonal Antibody
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$248.00 USD
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PDK2 Rabbit Polyclonal Antibody
Overview
| Product name: | PDK2 rabbit pAb |
| Alternative Names: | PDK2; PDHK2; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2; mitochondrial; Pyruvate dehydrogenase kinase isoform 2 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PDK2. AA range:260-309 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 46kD |
| GeneID(Human): | 5164 |
| Human Swiss-Prot No: | Q15119 |
| Cellular localization: | Mitochondrion matrix. |
| Background: | This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using PDK2 Polyclonal Antibody diluted at 1:500 -
Western blot analysis of lysates from HepG2 cells, using PDK2 Antibody. The lane on the right is blocked with the synthesized peptide. -
Western blot analysis of the lysates from HepG2 cells using PDK2 antibody. -
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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