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ELK Biotechnology
SKU:ES3168
PDK1 Rabbit Polyclonal Antibody
PDK1 Rabbit Polyclonal Antibody
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PDK1 Rabbit Polyclonal Antibody
Overview
| Product name: | PDK1 rabbit pAb |
| Alternative Names: | PDK1; PDHK1; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1; mitochondrial; Pyruvate dehydrogenase kinase isoform 1 |
| Applications: | WB;IHC |
| Recommended Dilutions: | WB 1:500-2000;IHC-p 1:50-300 |
| Immunogen: | Synthesized peptide derived from PDK1 . at AA range: 80-160 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 50kD |
| GeneID(Human): | 5163 |
| Human Swiss-Prot No: | Q15118 |
| Cellular localization: | Mitochondrion matrix . |
| Background: | Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using PDK1 Polyclonal Antibody -
Western Blot analysis of Hela cells using PDK1 Polyclonal Antibody -
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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