USO1; VDP; General vesicular transport factor p115; Protein USO1 homolog; Transcytosis-associated protein; TAP; Vesicle-docking protein
Applications:
WB;ELISA
Recommended Dilutions:
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human USO1. AA range:913-962
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
108kD
GeneID（Human）:
8615
Human Swiss-Prot No:
O60763
Cellular localization:
Cytoplasm, cytosol . Golgi apparatus membrane ; Peripheral membrane protein . Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes. .
Background:
The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014],
Western Blot analysis of various cells using p115 Polyclonal Antibody diluted at 1:2000
Western Blot analysis of K562 cells using p115 Polyclonal Antibody diluted at 1:2000
Western blot analysis of lysates from MCF-7, HeLa, and Jurkat cells, using USO1 Antibody. The lane on the right is blocked with the synthesized peptide.