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ELK Biotechnology
SKU:ES3083
OPN Rabbit Polyclonal Antibody
OPN Rabbit Polyclonal Antibody
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$248.00 USD
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OPN Rabbit Polyclonal Antibody
Overview
| Product name: | OPN rabbit pAb |
| Alternative Names: | SPP1; BNSP; OPN; PSEC0156; Osteopontin; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | WB 1:500-2000;IHC-p 1:100-500;IF/ICC 1:100-500;ELISA 1:5000-20000 |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Osteopontin. AA range:261-310 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 60kD |
| GeneID(Human): | 6696 |
| Human Swiss-Prot No: | P10451 |
| Cellular localization: | Secreted. |
| Background: | secreted phosphoprotein 1(SPP1) Homo sapiens The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using OPN Polyclonal Antibody diluted at 1:500 -
Western Blot analysis of 453 cells using OPN Polyclonal Antibody diluted at 1:500 -
Western blot analysis of lysates from LOVO cells, using Osteopontin Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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