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ELK Biotechnology
SKU:ES2942
NF-YC Rabbit Polyclonal Antibody
NF-YC Rabbit Polyclonal Antibody
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NF-YC Rabbit Polyclonal Antibody
Overview
| Product name: | NF-YC rabbit pAb |
| Alternative Names: | NFYC; Nuclear transcription factor Y subunit gamma; CAAT box DNA-binding protein subunit C; Nuclear transcription factor Y subunit C; NF-YC; Transactivator HSM-1/2 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human NFYC. AA range:1-50 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 50kD |
| GeneID(Human): | 4802 |
| Human Swiss-Prot No: | Q13952 |
| Cellular localization: | Nucleus. |
| Background: | This gene encodes one subunit of a trimeric complex forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoters of a variety of genes. The encoded protein, subunit C, forms a tight dimer with the B subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using NF-YC Polyclonal Antibody diluted at 1:1000 -
Western Blot analysis of 293 cells using NF-YC Polyclonal Antibody diluted at 1:1000 -
Western blot analysis of lysates from HeLa and 293 cells, using NFYC Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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