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ELK Biotechnology
SKU:ES2941
NF-YB Rabbit Polyclonal Antibody
NF-YB Rabbit Polyclonal Antibody
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NF-YB Rabbit Polyclonal Antibody
Overview
| Product name: | NF-YB rabbit pAb |
| Alternative Names: | NFYB; HAP3; Nuclear transcription factor Y subunit beta; CAAT box DNA-binding protein subunit B; Nuclear transcription factor Y subunit B; NF-YB |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human NFYB. AA range:1-50 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 29kD |
| GeneID(Human): | 4801 |
| Human Swiss-Prot No: | P25208 |
| Cellular localization: | Nucleus. |
| Background: | The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using NF-YB Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western blot analysis of lysates from 293 cells, using NFYB Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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