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ELK Biotechnology
SKU:ES2914
NEEP21 Rabbit Polyclonal Antibody
NEEP21 Rabbit Polyclonal Antibody
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NEEP21 Rabbit Polyclonal Antibody
Overview
| Product name: | NEEP21 rabbit pAb |
| Alternative Names: | NSG1; D4S234; Neuron-specific protein family member 1; Brain neuron cytoplasmic protein 1 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human NSG1. AA range:112-161 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 26kD |
| GeneID(Human): | 27065 |
| Human Swiss-Prot No: | P42857 |
| Cellular localization: | Membrane ; Single-pass type II membrane protein . Golgi apparatus, trans-Golgi network membrane . Endosome membrane . Cell projection, dendrite . Early endosome membrane . Late endosome membrane . Lysosome lumen . Recycling endosome membrane . Cytoplasmic vesicle membrane . Golgi apparatus, Golgi stack membrane . Endosome, multivesicular body membrane . Endoplasmic reticulum membrane . Endocytosed from the cell surface, thus enters into early endosomes, trafficks to late endosomes and degradates in lysosomes (By similarity). Endoplasmic reticulum targeting is essential for apoptosis (PubMed:20599942). Found in both stationary and motile endosomes. A previous study supports a type I membrane protein topology (By similarity). . |
| Background: | similarity:Belongs to the NSG family., |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using NEEP21 Polyclonal Antibody diluted at 1:2000 -
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i -
Western blot analysis of lysates from COLO cells, using NSG1 Antibody. The lane on the right is blocked with the synthesized peptide. -
Western blot analysis of the lysates from K562 cells using NSG1 antibody.
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