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ELK Biotechnology
SKU:ES2895
Nanog P8 Rabbit Polyclonal Antibody
Nanog P8 Rabbit Polyclonal Antibody
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$248.00 USD
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Nanog P8 Rabbit Polyclonal Antibody
Overview
| Product name: | Nanog P8 rabbit pAb |
| Alternative Names: | NANOGP8; Putative homeobox protein NANOGP8 |
| Applications: | WB;IF;ELISA;IHC |
| Recommended Dilutions: | WB 1:500-2000; IF/ICC 1:50-200;ELISA 1:2000-20000;IHC-p 1:50-200 |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human NANOGP8. AA range:51-100 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 35kD |
| GeneID(Human): | 388112 |
| Human Swiss-Prot No: | Q6NSW7 |
| Cellular localization: | Nucleus . |
| Background: | This locus is a processed pseudogene of the transcription factor NANOG. NANOG plays a central role in regulating self-renewal in pluripotent stem cells and tumor cells. This pseudogene contains an intact open reading frame that could potentially encode a protein similar to NANOG. Although there is no evidence of transcription from this pseudogene, RT-PCR studies suggest that NANOGP8 may be expressed in some cancer cell lines. In vitro studies using a recombinant NANOGP8 protein have shown that the protein localizes to the nucleus and can promote cell proliferation, similar to NANOG. [provided by RefSeq, Sep 2009], |
| Species Reactivity: | Human;Rat;Mouse; |
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Western Blot analysis of various cells using Nanog P8 Polyclonal Antibody diluted at 1:2000 -
Western blot analysis of lysates from HUVEC, HT-29, HepG2, and Jurkat cells, using NANOGP8 Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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