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ELK Biotechnology
SKU:ES2874
MYH Rabbit Polyclonal Antibody
MYH Rabbit Polyclonal Antibody
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$248.00 USD
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MYH Rabbit Polyclonal Antibody
Overview
| Product name: | MYH rabbit pAb |
| Alternative Names: | MUTYH; MYH; A/G-specific adenine DNA glycosylase; MutY homolog; hMYH |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human MUTYH. AA range:151-200 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 60kD |
| GeneID(Human): | 4595 |
| Human Swiss-Prot No: | Q9UIF7 |
| Cellular localization: | Nucleus . Mitochondrion . |
| Background: | This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. Mutations in this gene result in heritable predisposition to colon and stomach cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using MYH Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Immunofluorescence analysis of A549 cells, using MUTYH Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from HeLa and HepG2 cells, using MUTYH Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human Small intestinal stromal tumor. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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