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ELK Biotechnology
SKU:ES2852
MRP-S35 Rabbit Polyclonal Antibody
MRP-S35 Rabbit Polyclonal Antibody
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MRP-S35 Rabbit Polyclonal Antibody
Overview
| Product name: | MRP-S35 rabbit pAb |
| Alternative Names: | MRPS35; MRPS28; HDCMD11P; MDS023; PSEC0213; 28S ribosomal protein S35; mitochondrial; MRP-S35; S35mt; 28S ribosomal protein S28, mitochondrial; MRP-S28; S28mt |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human MRPS35. AA range:211-260 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 40kD |
| GeneID(Human): | 60488 |
| Human Swiss-Prot No: | P82673 |
| Cellular localization: | Mitochondrion . |
| Background: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has had confusing nomenclature in the literature. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on |
| Species Reactivity: | Human;Rat;Mouse; |
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Western Blot analysis of various cells using MRP-S35 Polyclonal Antibody -
Western Blot analysis of K562 cells using MRP-S35 Polyclonal Antibody -
Western blot analysis of lysates from Jurkat, COLO, and A549 cells, using MRPS35 Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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