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ELK Biotechnology
SKU:ES2851
MRP-S34 Rabbit Polyclonal Antibody
MRP-S34 Rabbit Polyclonal Antibody
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MRP-S34 Rabbit Polyclonal Antibody
Overview
| Product name: | MRP-S34 rabbit pAb |
| Alternative Names: | MRPS34; 28S ribosomal protein S34; mitochondrial; MRP-S34; S34mt |
| Applications: | WB;ELISA;IHC |
| Recommended Dilutions: | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human MRPS34. AA range:169-218 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 26kD |
| GeneID(Human): | 65993 |
| Human Swiss-Prot No: | P82930 |
| Cellular localization: | Mitochondrion . |
| Background: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014], |
| Species Reactivity: | Human;Mouse |
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Western Blot analysis of various cells using MRP-S34 Polyclonal Antibody diluted at 1:1000 -
Western Blot analysis of Jurkat cells using MRP-S34 Polyclonal Antibody diluted at 1:1000 -
Western blot analysis of lysates from HeLa, HUVEC, HT-29, A549, and Jurkat cells, using MRPS34 Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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