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ELK Biotechnology
SKU:ES2848
MRP-S22 Rabbit Polyclonal Antibody
MRP-S22 Rabbit Polyclonal Antibody
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MRP-S22 Rabbit Polyclonal Antibody
Overview
| Product name: | MRP-S22 rabbit pAb |
| Alternative Names: | MRPS22; C3orf5; RPMS22; GK002; 28S ribosomal protein S22; mitochondrial; MRP-S22; S22mt |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human MRPS22. AA range:231-280 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 41kD |
| GeneID(Human): | 56945 |
| Human Swiss-Prot No: | P82650 |
| Cellular localization: | Mitochondrion . |
| Background: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that does not seem to have a counterpart in prokaryotic and fungal-mitochondrial ribosomes. This gene lies telomeric of and is transcribed in the opposite direction from the forkhead box L2 gene. A pseudogene |
| Species Reactivity: | Human;Monkey;Bovine;Hamster;Cow |
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Western Blot analysis of various cells using MRP-S22 Polyclonal Antibody -
Western blot analysis of mouse-brain 293T lysis using MRP-S22 antibody. -
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-abs -
Immunofluorescence analysis of A549 cells, using MRPS22 Antibody. The picture on the right is blocked with the synthesized peptide.
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