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ELK Biotechnology
SKU:ES2843
MRP-L50 Rabbit Polyclonal Antibody
MRP-L50 Rabbit Polyclonal Antibody
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MRP-L50 Rabbit Polyclonal Antibody
Overview
| Product name: | MRP-L50 rabbit pAb |
| Alternative Names: | MRPL50; 39S ribosomal protein L50; mitochondrial; L50mt; MRP-L50 |
| Applications: | WB;ELISA;IHC |
| Recommended Dilutions: | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human MRPL50. AA range:109-158 |
| Storage: | -20°C/1 year |
| Storage: | Rabbit |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 22kD |
| GeneID(Human): | 54534 |
| Human Swiss-Prot No: | Q8N5N7 |
| Cellular localization: | Mitochondrion . |
| Background: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a putative 39S subunit protein and belongs to the L47P ribosomal protein family. Pseudogenes corresponding to this gene are found on chromosomes 2p, 2q, 5p, and 10q. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Rat;Mouse; |
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Western Blot analysis of various cells using MRP-L50 Polyclonal Antibody -
Western blot analysis of lysates from COLO and HT-29 cells, using MRPL50 Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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