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ELK Biotechnology
SKU:ES2838
MRP-L4 Rabbit Polyclonal Antibody
MRP-L4 Rabbit Polyclonal Antibody
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MRP-L4 Rabbit Polyclonal Antibody
Overview
| Product name: | MRP-L4 rabbit pAb |
| Alternative Names: | MRPL4; CDABP0091; CGI-28; 39S ribosomal protein L4; mitochondrial; L4mt; MRP-L4 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human MRPL4. AA range:90-139 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 35kD |
| GeneID(Human): | 51073 |
| Human Swiss-Prot No: | Q9BYD3 |
| Cellular localization: | Mitochondrion . |
| Background: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified alternatively spliced variants that encode different protein isoforms. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse |
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Western Blot analysis of various cells using MRP-L4 Polyclonal Antibody -
Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbe -
Western blot analysis of lysates from COLO cells, using MRPL4 Antibody. The lane on the right is blocked with the synthesized peptide. -
Western blot analysis of the lysates from HeLa cells using MRPL4 antibody.
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