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ELK Biotechnology
SKU:ES2831
MRP-L15 Rabbit Polyclonal Antibody
MRP-L15 Rabbit Polyclonal Antibody
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MRP-L15 Rabbit Polyclonal Antibody
Overview
| Product name: | MRP-L15 rabbit pAb |
| Alternative Names: | MRPL15; HSPC145; 39S ribosomal protein L15; mitochondrial; L15mt; MRP-L15 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human MRPL15. AA range:191-240 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 33kD |
| GeneID(Human): | 29088 |
| Human Swiss-Prot No: | Q9P015 |
| Cellular localization: | Mitochondrion . |
| Background: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL15 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 15q. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse |
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Western Blot analysis of various cells using MRP-L15 Polyclonal Antibody -
Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbe -
Western blot analysis of lysates from HeLa, HUVEC, and MCF-7 cells, using MRPL15 Antibody. The lane on the right is blocked with the synthesized peptide.
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