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ELK Biotechnology
SKU:ES2826
MRP-L10 Rabbit Polyclonal Antibody
MRP-L10 Rabbit Polyclonal Antibody
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MRP-L10 Rabbit Polyclonal Antibody
Overview
| Product name: | MRP-L10 rabbit pAb |
| Alternative Names: | MRPL10; MRPL8; RPML8; 39S ribosomal protein L10; mitochondrial; L10mt; MRP-L10; 39S ribosomal protein L8, mitochondrial; L8mt; MRP-L8 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human MRPL10. AA range:211-260 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 29kD |
| GeneID(Human): | 124995 |
| Human Swiss-Prot No: | Q7Z7H8 |
| Cellular localization: | Mitochondrion . |
| Background: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants that encode two different isoforms. A pseudogene corresponding to this gene is found on chromosome 5q. [provided by RefSeq, Nov 2010], |
| Species Reactivity: | Human;Rat;Mouse; |
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Western Blot analysis of various cells using MRP-L10 Polyclonal Antibody diluted at 1:1000 -
Western blot analysis of 293T lysis using MRP-L10 antibody. Antibody was diluted at 1:1000 -
Western blot analysis of lysates from 293, HepG2, and COLO cells, using MRPL10 Antibody. The lane on the right is blocked with the synthesized peptide. -
Western blot analysis of the lysates from HT-29 cells using MRPL10 antibody.
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