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ELK Biotechnology
SKU:ES2799
MLK4 Rabbit Polyclonal Antibody
MLK4 Rabbit Polyclonal Antibody
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$248.00 USD
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MLK4 Rabbit Polyclonal Antibody
Overview
| Product name: | MLK4 rabbit pAb |
| Alternative Names: | MLK4; KIAA1804; Mitogen-activated protein kinase kinase kinase MLK4; Mixed lineage kinase 4 |
| Applications: | WB;ELISA;IHC |
| Recommended Dilutions: | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human MAP3KL4. AA range:631-680 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 110kD |
| GeneID(Human): | 84451 |
| Human Swiss-Prot No: | Q5TCX8 |
| Cellular localization: | intracellular, |
| Background: | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation.,function:Activates the JUN N-terminal pathway.,PTM:Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH3 domain.,subunit:Homodimer., |
| Species Reactivity: | Human;Rat;Mouse; |
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Western Blot analysis of various cells using MLK4 Polyclonal Antibody -
Western blot analysis of lysates from HT-29 cells, using MAP3KL4 Antibody. The lane on the right is blocked with the synthesized peptide. -
Western blot analysis of the lysates from HepG2 cells using MAP3KL4 antibody. -
Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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