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ELK Biotechnology
SKU:ES2797
MLH1 Rabbit Polyclonal Antibody
MLH1 Rabbit Polyclonal Antibody
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$248.00 USD
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MLH1 Rabbit Polyclonal Antibody
Overview
| Product name: | MLH1 rabbit pAb |
| Alternative Names: | MLH1; COCA2; DNA mismatch repair protein Mlh1; MutL protein homolog 1 |
| Applications: | WB;IHC |
| Recommended Dilutions: | WB 1:500-2000;IHC-p 1:50-300 |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human MLH1. AA range:441-490 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 85kD |
| GeneID(Human): | 4292 |
| Human Swiss-Prot No: | P40692 |
| Cellular localization: | Nucleus . Chromosome . Recruited to chromatin in a MCM9-dependent manner. . |
| Background: | This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.[provided by RefSeq, Nov 2009], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using MLH1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western Blot analysis of Jurkat cells using MLH1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western blot analysis of lysates from K562 cells, using MLH1 Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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