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ELK Biotechnology
SKU:ES2583
HSP40 Rabbit Polyclonal Antibody
HSP40 Rabbit Polyclonal Antibody
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$248.00 USD
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HSP40 Rabbit Polyclonal Antibody
Overview
| Product name: | HSP40 rabbit pAb |
| Alternative Names: | DNAJB1; DNAJ1; HDJ1; HSPF1; DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; Heat shock 40 kDa protein 1; HSP40; Heat shock protein 40; Human DnaJ protein 1; hDj-1 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human HSP40. AA range:271-320 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 45kD |
| GeneID(Human): | 3337 |
| Human Swiss-Prot No: | P25685 |
| Cellular localization: | Cytoplasm . Nucleus . Nucleus, nucleolus . Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock. |
| Background: | This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015], |
| Species Reactivity: | Human;Mouse;Rat |
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Immunofluorescence analysis of Hela cell. 1,HSP40 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Caspase-8 Monoclonal Antibody(2G12)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at -
Western Blot analysis of various cells using HSP40 Polyclonal Antibody -
Immunofluorescence analysis of NIH/3T3 cells, using HSP40 Antibody. The picture on the right is blocked with the synthesized peptide. -
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using HSP40 Antibody. The picture on the right is blocked with the synthesized peptide.
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