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ELK Biotechnology
SKU:ES2562
HoxD12 Rabbit Polyclonal Antibody
HoxD12 Rabbit Polyclonal Antibody
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$248.00 USD
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HoxD12 Rabbit Polyclonal Antibody
Overview
| Product name: | HoxD12 rabbit pAb |
| Alternative Names: | HOXD12; HOX4H; Homeobox protein Hox-D12; Homeobox protein Hox-4H |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human HOXD12. AA range:191-240 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 28kD |
| GeneID(Human): | 3238 |
| Human Swiss-Prot No: | P35452 |
| Cellular localization: | Nucleus. |
| Background: | This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located in a cluster on chromosome 2. Deletions that remove the entire HOXD gene cluster or the 5' end of this cluster have been associated with severe limb and genital abnormalities. The exact role of this gene has not been determined. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse |
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Western Blot analysis of various cells using HoxD12 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absor -
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absor -
Western blot analysis of lysates from COLO205 and HucEc cells, treated with serum 20% 15', using HOXD12 Antibody. The lane on the right is blocked with the synthesized peptide.
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