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ELK Biotechnology
SKU:ES2541
HNF-4α Rabbit Polyclonal Antibody
HNF-4α Rabbit Polyclonal Antibody
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HNF-4α Rabbit Polyclonal Antibody
Overview
| Product name: | HNF-4α rabbit pAb |
| Alternative Names: | HNF4A; HNF4; NR2A1; TCF14; Hepatocyte nuclear factor 4-alpha; HNF-4-alpha; Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; TCF-14; Transcription factor HNF-4 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human HNF4 alpha. AA range:280-329 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 52kD |
| GeneID(Human): | 3172 |
| Human Swiss-Prot No: | P41235 |
| Cellular localization: | Nucleus. |
| Background: | The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using HNF-4α Polyclonal Antibody diluted at 1:2000 -
Western blot analysis of lysates from HT-29 cells, using HNF4 alpha Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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