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ELK Biotechnology
SKU:ES2525
Histone H2A.X Rabbit Polyclonal Antibody
Histone H2A.X Rabbit Polyclonal Antibody
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Histone H2A.X Rabbit Polyclonal Antibody
Overview
| Product name: | Histone H2A.X rabbit pAb |
| Alternative Names: | H2AFX; H2AX; Histone H2A.x; H2a/x |
| Applications: | WB;IHC;IF;CoIP;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. IP 1:200-500,ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Histone H2A.X. AA range:94-143 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 19kD |
| GeneID(Human): | 3014 |
| Human Swiss-Prot No: | P16104 |
| Cellular localization: | Nucleus . Chromosome . |
| Background: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015], |
| Species Reactivity: | Human;Mouse;Rat |
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Immunofluorescence analysis of Hela cell. 1,Histone H2A.X Polyclonal Antibody(red) was diluted at 1:200(4° overnight). LC3B Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1: -
Immunofluorescence analysis of rat-lung tissue. 1,Histone H2A.X Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. -
Immunofluorescence analysis of rat-spleen tissue. 1,Histone H2A.X Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target -
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,Histone H2A.X Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:20
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