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ELK Biotechnology
SKU:ES2495
HBP1 Rabbit Polyclonal Antibody
HBP1 Rabbit Polyclonal Antibody
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$248.00 USD
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HBP1 Rabbit Polyclonal Antibody
Overview
| Product name: | HBP1 rabbit pAb |
| Alternative Names: | HBP1; HMG box-containing protein 1; HMG box transcription factor 1; High mobility group box transcription factor 1 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human HBP1. AA range:431-480 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 57kD |
| GeneID(Human): | 26959 |
| Human Swiss-Prot No: | O60381 |
| Cellular localization: | Nucleus . |
| Background: | function:Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the H1F0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4.,similarity:Contains 1 AXH domain.,similarity:Contains 1 HMG box DNA-binding domain.,subunit:Binds the second PAH repeat of SIN3A (By similarity). Binds TCF4 and RB1., |
| Species Reactivity: | Human;Mouse;Rat;Monkey |
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Western Blot analysis of various cells using HBP1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western blot analysis of lysates from COS7 cells, using HBP1 Antibody. The lane on the right is blocked with the synthesized peptide. -
Western blot analysis of the lysates from 293 cells using HBP1 antibody. -
Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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