Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human GPR120. AA range:221-270
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
38kD
GeneID（Human）:
338557
Human Swiss-Prot No:
Q5NUL3
Cellular localization:
[Isoform 1]: Cell membrane ; Multi-pass membrane protein . Endosome membrane ; Multi-pass membrane protein . Lysosome membrane ; Multi-pass membrane protein . Sorted to late endosome/lysosome compartments upon internalization. .; [Isoform 2]: Cell membrane ; Multi-pass membrane protein . Endosome membrane ; Multi-pass membrane protein . Lysosome membrane ; Multi-pass membrane protein . Cell projection, cilium membrane ; Multi-pass membrane protein . Sorted to late endosome/lysosome compartments upon internalization (PubMed:22282525). Specifically localizes to the primary cilium of undifferentiated adipocytes. Ciliary trafficking is TULP3-dependent. As the cilium is lost during adipogenesis, moves to the plasma membrane (Probable). .
Background:
This gene encodes a G protein-coupled receptor (GPR) which belongs to the rhodopsin family of GPRs. The encoded protein functions as a receptor for free fatty acids, including omega-3, and participates in suppressing anti-inflammatory responses and insulin sensitizing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012],
Western Blot analysis of various cells using GPR120 Polyclonal Antibody diluted at 1:500
Western Blot analysis of HEPG2-UV cells using GPR120 Polyclonal Antibody diluted at 1:500
Immunofluorescence analysis of LOVO cells, using GPR120 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from LOVO cells, using GPR120 Antibody. The lane on the right is blocked with the synthesized peptide.