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ELK Biotechnology
SKU:ES2407
GCG Rabbit Polyclonal Antibody
GCG Rabbit Polyclonal Antibody
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$248.00 USD
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GCG Rabbit Polyclonal Antibody
Overview
| Product name: | GCG rabbit pAb |
| Alternative Names: | GCG; Glucagon |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Glucagon. AA range:61-110 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 25kD |
| GeneID(Human): | 2641 |
| Human Swiss-Prot No: | P01275 |
| Cellular localization: | Secreted .; [Glucagon-like peptide 1]: Secreted . |
| Background: | The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat;Monkey |
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Western Blot analysis of various cells using GCG Polyclonal Antibody -
Immunofluorescence analysis of NIH/3T3 cells, using Glucagon Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from COS7 cells, using Glucagon Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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