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ELK Biotechnology
SKU:ES2383
G2A Rabbit Polyclonal Antibody
G2A Rabbit Polyclonal Antibody
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$248.00 USD
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G2A Rabbit Polyclonal Antibody
Overview
| Product name: | G2A rabbit pAb |
| Alternative Names: | GPR132; G2A; Probable G-protein coupled receptor 132; G2 accumulation protein |
| Applications: | WB;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human GPR132. AA range:293-342 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 42kD |
| GeneID(Human): | 29933 |
| Human Swiss-Prot No: | Q9UNW8 |
| Cellular localization: | Cell membrane ; Multi-pass membrane protein . Internalized and accumulated in endosomal compartments. LPC triggers the relocalization from the endosomal compartment to the cell surface (By similarity). . |
| Background: | This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein was reported to be a receptor for lysophosphatidylcholine action, but PubMedID: 15653487 retracts this finding and instead suggests this protein to be an effector of lysophosphatidylcholine action. This protein may have proton-sensing activity and may be a receptor for oxidized free fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013], |
| Species Reactivity: | Human;Mouse;Monkey |
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Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min. -
Western Blot analysis of various cells using G2A Polyclonal Antibody diluted at 1:500 -
Western Blot analysis of 293T cells using G2A Polyclonal Antibody diluted at 1:500 -
Western blot analysis of lysates from COS7 cells, using GPR132 Antibody. The lane on the right is blocked with the synthesized peptide.
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