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ELK Biotechnology
SKU:ES2359
Fos B Rabbit Polyclonal Antibody
Fos B Rabbit Polyclonal Antibody
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$248.00 USD
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Fos B Rabbit Polyclonal Antibody
Overview
| Product name: | Fos B rabbit pAb |
| Alternative Names: | FOSB; G0S3; Protein fosB; G0/G1 switch regulatory protein 3 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human FosB. AA range:12-61 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 35kD |
| GeneID(Human): | 2354 |
| Human Swiss-Prot No: | P53539 |
| Cellular localization: | Nucleus . |
| Background: | The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Monkey |
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Western Blot analysis of various cells using Fos B Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Immunofluorescence analysis of HepG2 cells, using FosB Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from COS7 and Jurkat cells, using FosB Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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