Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human FBLN5. AA range:171-220
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
50kD
GeneID（Human）:
10516
Human Swiss-Prot No:
Q9UBX5
Cellular localization:
Secreted . Secreted, extracellular space, extracellular matrix . co-localizes with ELN in elastic fibers. .
Background:
The protein encoded by this gene is a secreted, extracellular matrix protein containing an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. It is prominently expressed in developing arteries but less so in adult vessels. However, its expression is reinduced in balloon-injured vessels and atherosclerotic lesions, notably in intimal vascular smooth muscle cells and endothelial cells. Therefore, the protein encoded by this gene may play a role in vascular development and remodeling. Defects in this gene are a cause of autosomal dominant cutis laxa, autosomal recessive cutis laxa type I (CL type I), and age-related macular degeneration type 3 (ARMD3). [provided by RefSeq, Jul 2008],
Western Blot analysis of various cells using Fibulin-5 Polyclonal Antibody diluted at 1:1000
Western Blot analysis of L929 cells using Fibulin-5 Polyclonal Antibody diluted at 1:1000
Western blot analysis of lysates from NIH/3T3 cells, using FBLN5 Antibody. The lane on the right is blocked with the synthesized peptide.